Health Network Laboratories Lab Handbook Search Page

Print

Neisseria gonorrhoeae, Amplified DNA Probe

CPT:

87591

Alternate Name:

GC Amplified DNA;

Methodology:

GEN-PROBE® APTIMA™ Amplified DNA Probe

Testing Schedule:

Routine Daily, Monday-Friday

Report Available:

1-4 days

Specimen Requirements:

Minimum Volume:

1 swab from urethra OR 1 swab from endocervical canal OR 20-30 mL initial urine stream OR ThinPrep® Liquid Pap specimen.ThinPrep® Liquid Pap specimens go directly to Cytology Registration.

Container:

APTIMA™ transport swab OR GEN-PROBE® APTIMA™ urine collection container

Collection:

•Follow instructions on collection kit package using ONLY the enclosed swab.
•Endocervical sampling for N. gonorrhoeae and/or C. trachomatis: Remove excess mucus from cervical os and surrounding mucosa using the white swab provided.Discard this swab.Insert the blue swab from collection kit 1-1.5 cm into endocervical canal.Gently rotate swab clockwise for 10-30 seconds in endocervical canal to ensure adequate sampling.Withdraw swab carefully; avoid any contact with vaginal mucosa.Remove the cap from the swab specimen transport tube and immediately place the specimen collection swab into the transport tube.Carefully break the swab shaft at the scoreline; use care to avoid splashing of contents. Re-cap the swab specimen transport tube tightly.
•Urethral sampling for C. trachomatis and/or N. gonorrhoeae: Patient should not have urinated for at least 1 hour prior to sampling.Insert the blue swab 2-4 cm into urethra, Gently rotate clockwise for 2-3 seconds to ensure contact with all urethral surfaces.Withdraw swab.Remove the cap from the swab specimen transport tube and immediately place the specimen collection swab into the transport tube.Carefully break the swab shaft at the scoreline; use care to avoid splashing of contents. Recap the swab specimen transport tube tightly.
•Urine: Patient should not have urinated for at least 1 hour prior to specimen collection.
•Direct patient to provide first-catch urine (approximately 20 to 30 mL of initial urine stream) into a clean, dry container free of any preservative.Collection of larger volumes of urine may result in specimen dilution and may reduce test sensitivity.Female patients should not cleanse labial area prior to providing specimen.
•Transport tube and transfer 2 mL of urine into specimen transport tube using disposable pipette provided.The correct volume of urine has been added when the fluid level is between black fill lines on the urine specimen transport tube label.
•Tightly re-cap the urine specimen transport tube.
•Urine specimens must be maintained at 2 C to 30 C in urine transport tube.
•ThinPrep® Liquid Pap Specimens should be collected in the routine manner.Specimens must be run within 14 days of collection.

Special Instructions and/or Comments:

•DO NOT use for legal specimens, rectal, conjunctival or nasopharyngeal specimens.For these specimens, submit GC Culture.
•Use only for urethral, endocervical and urine specimens.
•Transport at room temperature or refrigerated.

Reference Range:

Negative for Neisseria gonorrhoeae by Amplified DNA Probe.

Clinical Utility:

To aid in the assessment of sexually transmitted infections.