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Dilute Russell’s Viper Venom Time

Includes:

•Dilute Russell’s Viper Venom Time (dRVVT) Screen
•Reflexed when appropriate:
•dRVVT Confirm on prolonged screen results

CPT:

85613

Alternate Name:

dRVVT; Russell’s Viper Venom Time

Methodology:

Clot Based Assay

Testing Schedule:

Routine, 1 time per week

Report Available:

5-7 days

Specimen Requirements:

Minimum Volume:

2 mL prepared “platelet poor plasma”, split equally into 2 plastic aliquot tubes and FROZEN immediately

Container:

2 blue top (sodium citrate) tubes

Collection:

See special handling instructions for “Coagulation Studies”, listed under Specimen Collection, reparation, and Handling

Special Instructions and/or Comments:

•Testing is contraindicated for patients on Heparin or Coumadin therapy.
•Preparation and submission of “platelet poor plasma” is critical for accurate test performance and interpretation.See collection note above.
Causes for rejection:
•Extended transport time to laboratory
•Clotted specimens
•Hemolyzed specimens
•Blue top sodium citrate tubes insufficiently filled.
•Aliquots not received frozen on dry ice
•Frozen aliquots not clearly identified as “platelet
poor plasma”

Reference Range:

Literature:
•Screen:...............28-50 seconds
•Confirm:............<1.3 seconds
•Specific in-house ranges are developed with each new reagent lot number and these are noted on patient report.
•A normal or negative dRVVT screen shows no evidence of LUA in the dRVVT assay.A negative result does not necessarily rule out the presence of an LUA or an APL antibody.Due to the heterogeneity associated with lupus anticoagulants, performance of two or more different screening assays may significantly enhance LUA detection.
•A positive dRVVT screen with positive reflex confirmation indicates that the presence of LUA is suggested by the dRVVT.Results should be interpreted in light of clinical history and in conjunction with other laboratory findings.

Clinical Utility:

The presence of Lupus Anticoagulant can cause hypercoagulable states, as well as fetal loss. Due to its heterogeneous nature, no single assay can absolutely identify the presence of LA in every test plasma. Other LA tests should be performed if DRVVT is negative.